In Vitro Cytotoxicity and Cell Seeding Studies of a Chitosan-silver Composite for Potential Wound Management Applications
نویسندگان
چکیده
There have been concerns raised over the treatment of acute burns with silver particles because of the potential toxicity caused by silver. As the wound heals, a membrane is formed between the silver particle and the blood, but this membrane is not impenetrable, which might lead to further silver absorption. This paper reports the in vitro cytotoxicity of silver-chitosan composites prepared as films and paste with various concentration of chitosan and a fixed concentration of 3 w/v % of silver nitrate, AgNO3. UV photo reduction was used to convert the silver ions (Ag) to silver particles, and the effect of adding silver to chitosan was thoroughly studied. Cell attachment studies using Normal Human Dermal Fibroblasts (NHDFs) are being carried out, and more evidence for the reliability of using silver as an antimicrobial agent in chitosan composites for wound management applications could be gained.
منابع مشابه
Development of antimicrobial chitosan based nanofiber dressings for wound healing applications
Objective(s): Chitosan based composite fine fibers were successfully produced via a centrifugal spinning technology. This study evaluates the ability of the composites to function as scaffolds for cell growth while maintaining an antibacterial activity. Materials and methods: Two sets of chitosan fiber composites were prepared, one filled with anti-microbial silver nanoparticles and another on...
متن کاملPreparation of tissue-engineered wound dressing consisting of chitosan fibers containing silver ion-doped bioactive nanoparticles for wound healing
Background: Wound healing is a complicated process involving the proliferation of the epithelial cells, deposition of granulation tissue as well as recruitment of inflammatory cells. It also is a hot topic of research for trauma, orthopedics and general surgery studies. There are many forms of cells involved in this process. This study aimed to design a tissue-engineered wound dressing consisti...
متن کاملWound dressing application of chitosan based bioactive compounds
Generation of immense quantity of underutilized marine processing byproducts has long been recognized as wastes and greater efforts were given to use these materials in various applications. With a great number of researches on these byproducts, some biologically active compounds were identified and implicated to the useful compounds for human utilization. Chitosan and silver nanoparticle have ...
متن کاملPreparation, characterization and antimicrobial property of ag+- nano Chitosan/ZSM-5: novel Hybrid Biocomposites
Objective(s): Binary hybrids of chitosan-zeolite have many interesting applications in separation and bacteriostatic activity. Materials and Methods: Template free ZSM-5 zeolite was synthesized by hydrothermal method, physical hydrogels of nano chitosan in the colloidal domain were obtained in the absence of toxic organic solvent and then nano chitosan/ZSM-5 hybrid composites with nano chitosan...
متن کاملNanoparticles Prepared From N,N-Dimethyl-N-Octyl Chitosan as the Novel Approach for Oral Delivery of Insulin: Preparation, Statistical Optimization and Characterization
In this study, N,N-Dimethyl-N-Octyl chitosan was synthesized. Nanoparticles containing insulin were prepared using PEC method and were statistically optimized using the Box-Behnken response surface methodology. The independent factors were considered to be the insulin concentration, concentration and pH of the polymer solution, while the dependent factors were characterized as the size, zeta po...
متن کامل